Regulation of the ald gene encoding alanine dehydrogenase by AldR 1 ( MSMEG _ 2660 ) in Mycobacterium smegmatis
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چکیده
Regulation of the ald gene encoding alanine dehydrogenase by AldR 1 (MSMEG_2660) in Mycobacterium smegmatis 2 3 Ji-A Jeong, Eun-Young Baek, Si Wouk Kim, Jong-Soon Choi, and Jeong-Il Oh 4 5 a Department of Microbiology, Pusan National University, 609-735 Busan, Korea 6 b Department of Environmental Engineering, Chosun University, 501-759 Gwangju, Korea 7 c Division of Life Science, Korea Basic Science Institute, 305-333 Daejeon, Korea 8 9 10 Corresponding author 11 Jeong-Il Oh 12 Telephone: +82-51-510-2593 13 Fax: +82-51-514-1778 14 E-mail: [email protected] 15 16 17 Running title: Regulation of the alanine dehydrogenase gene 18 19 Copyright © 2013, American Society for Microbiology. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.00482-13 JB Accepts, published online ahead of print on 7 June 2013
منابع مشابه
Mycobacterium smegmatis L-alanine dehydrogenase (Ald) is required for proficient utilization of alanine as a sole nitrogen source and sustained anaerobic growth.
NAD(H)-dependent L-alanine dehydrogenase (EC 1.4.1.1) (Ald) catalyzes the oxidative deamination of L-alanine and the reductive amination of pyruvate. To assess the physiological role of Ald in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression levels, and analyzed the combined effects of nutrient supplementation, oxygen availability, and ...
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